Construct: shRNA TRCN0000325541

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008542.2-1179s21c1
DNA Barcode:
GTCCGATTCTACATTGTCTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Smad6 (17130)
Gene Description:
SMAD family member 6
Transcript:
RefSeq NM_008542.2 (NON-CURRENT)
Match location:
Position 1179 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325541)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTCCGATTCTACATTGTCTTA
Hairpin Sequence:
5'-CCGG-GTCCGATTCTACATTGTCTTA-CTCGAG-TAAGACAATGTAGAATCGGAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTCCGATTCTACATTGTCTTACTCGAGTAAGACAATGTAGAATCGGACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTCCGATTCTACATTGTCTTACTCGAGTAAGACAATGTAGAATCGGAC-3'

Other clones with same target sequence:

  1. TRCN0000089525