Construct: shRNA TRCN0000325587

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009798.3-843s21c1
DNA Barcode:
GCAGACAAATCAAAGCAAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Capzb (12345)
Gene Description:
capping protein (actin filament) muscle Z-line, beta
Transcript:
RefSeq NM_009798.3 (NON-CURRENT)
Match location:
Position 843 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325587)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGACAAATCAAAGCAAGAA
Hairpin Sequence:
5'-CCGG-GCAGACAAATCAAAGCAAGAA-CTCGAG-TTCTTGCTTTGATTTGTCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGACAAATCAAAGCAAGAACTCGAGTTCTTGCTTTGATTTGTCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGACAAATCAAAGCAAGAACTCGAGTTCTTGCTTTGATTTGTCTGC-3'

Other clones with same target sequence:

  1. TRCN0000091884