Construct: shRNA TRCN0000325674

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175193.5-845s21c1
DNA Barcode:
GCAAGATTCCAATAGCAGATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Golim4 (73124)
Gene Description:
golgi integral membrane protein 4
Transcript:
RefSeq NM_175193.5 (NON-CURRENT)
Match location:
Position 845 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325674)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAAGATTCCAATAGCAGATA
Hairpin Sequence:
5'-CCGG-GCAAGATTCCAATAGCAGATA-CTCGAG-TATCTGCTATTGGAATCTTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAAGATTCCAATAGCAGATACTCGAGTATCTGCTATTGGAATCTTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAAGATTCCAATAGCAGATACTCGAGTATCTGCTATTGGAATCTTGC-3'

Other clones with same target sequence:

  1. TRCN0000126036