Construct: shRNA TRCN0000325695

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008784.2-181s21c1
DNA Barcode:
GCTGGAATGTTATCGCAGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Igbp1 (18518)
Gene Description:
immunoglobulin (CD79A) binding protein 1
Transcript:
RefSeq NM_008784.2 (NON-CURRENT)
Match location:
Position 181 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325695)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAATGTTATCGCAGCTT
Hairpin Sequence:
5'-CCGG-GCTGGAATGTTATCGCAGCTT-CTCGAG-AAGCTGCGATAACATTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAATGTTATCGCAGCTTCTCGAGAAGCTGCGATAACATTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAATGTTATCGCAGCTTCTCGAGAAGCTGCGATAACATTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000067186