Construct: shRNA TRCN0000325733

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133869.3-1586s21c1
DNA Barcode:
CCCATCCTATAAACTGAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cept1 (99712)
Gene Description:
choline/ethanolaminephosphotransferase 1
Transcript:
RefSeq NM_133869.3 (NON-CURRENT)
Match location:
Position 1586 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325733)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCATCCTATAAACTGAATAT
Hairpin Sequence:
5'-CCGG-CCCATCCTATAAACTGAATAT-CTCGAG-ATATTCAGTTTATAGGATGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCATCCTATAAACTGAATATCTCGAGATATTCAGTTTATAGGATGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCATCCTATAAACTGAATATCTCGAGATATTCAGTTTATAGGATGGG-3'

Other clones with same target sequence:

  1. TRCN0000103315