Construct: shRNA TRCN0000325793

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026298.4-4704s21c1
DNA Barcode:
GCTGCTGATCTCTCATTACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ift172 (67661)
Gene Description:
intraflagellar transport 172
Transcript:
RefSeq NM_026298.4 (NON-CURRENT)
Match location:
Position 4704 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325793)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGCTGATCTCTCATTACTA
Hairpin Sequence:
5'-CCGG-GCTGCTGATCTCTCATTACTA-CTCGAG-TAGTAATGAGAGATCAGCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGCTGATCTCTCATTACTACTCGAGTAGTAATGAGAGATCAGCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGCTGATCTCTCATTACTACTCGAGTAGTAATGAGAGATCAGCAGC-3'

Other clones with same target sequence:

  1. TRCN0000079814