Construct: shRNA TRCN0000325849

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008797.2-1453s21c1
DNA Barcode:
CCGAGGTGTAAAGACCAACAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Pcx (18563)
Gene Description:
pyruvate carboxylase
Transcript:
RefSeq NM_008797.2 (NON-CURRENT)
Match location:
Position 1453 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325849)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGAGGTGTAAAGACCAACAT
Hairpin Sequence:
5'-CCGG-CCGAGGTGTAAAGACCAACAT-CTCGAG-ATGTTGGTCTTTACACCTCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGAGGTGTAAAGACCAACATCTCGAGATGTTGGTCTTTACACCTCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGAGGTGTAAAGACCAACATCTCGAGATGTTGGTCTTTACACCTCGG-3'

Other clones with same target sequence:

  1. TRCN0000112426