Construct: shRNA TRCN0000325859

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_012000.3-1111s21c1
DNA Barcode:
CACCACCTCTTTGCCTTTCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cln8 (26889)
Gene Description:
ceroid-lipofuscinosis, neuronal 8
Transcript:
RefSeq NM_012000.3 (CURRENT)
Match location:
Position 1111 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325859)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CACCACCTCTTTGCCTTTCTT
Hairpin Sequence:
5'-CCGG-CACCACCTCTTTGCCTTTCTT-CTCGAG-AAGAAAGGCAAAGAGGTGGTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCACCACCTCTTTGCCTTTCTTCTCGAGAAGAAAGGCAAAGAGGTGGTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACACCACCTCTTTGCCTTTCTTCTCGAGAAGAAAGGCAAAGAGGTGGTG-3'

Other clones with same target sequence:

  1. TRCN0000105004