Construct: shRNA TRCN0000325890

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010593.1-1598s21c1
DNA Barcode:
CCACTGGTCAAGGCAACTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Jup (16480)
Gene Description:
junction plakoglobin
Transcript:
RefSeq NM_010593.1 (NON-CURRENT)
Match location:
Position 1598 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325890)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCACTGGTCAAGGCAACTATT
Hairpin Sequence:
5'-CCGG-CCACTGGTCAAGGCAACTATT-CTCGAG-AATAGTTGCCTTGACCAGTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCACTGGTCAAGGCAACTATTCTCGAGAATAGTTGCCTTGACCAGTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCACTGGTCAAGGCAACTATTCTCGAGAATAGTTGCCTTGACCAGTGG-3'

Other clones with same target sequence:

  1. TRCN0000089919