Construct: shRNA TRCN0000325912
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_008810.2-630s21c1
- DNA Barcode:
- TCAGATCTTTGAAGCTTACAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Pdha1 (18597)
- Gene Description:
- pyruvate dehydrogenase E1 alpha 1
- Transcript:
- RefSeq NM_008810.2 (NON-CURRENT)
- Match location:
- Position 630 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000325912)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- TCAGATCTTTGAAGCTTACAA
- Hairpin Sequence:
-
5'-CCGG-TCAGATCTTTGAAGCTTACAA-CTCGAG-TTGTAAGCTTCAAAGATCTGA-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGTCAGATCTTTGAAGCTTACAACTCGAGTTGTAAGCTTCAAAGATCTGATTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAATCAGATCTTTGAAGCTTACAACTCGAGTTGTAAGCTTCAAAGATCTGA-3'
Other clones with same target sequence:
-
TRCN0000041917
