Construct: shRNA TRCN0000325938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008214.3-1613s21c1
DNA Barcode:
GTACTTAAACAGTCTGCATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Hars (15115)
Gene Description:
histidyl-tRNA synthetase
Transcript:
RefSeq NM_008214.3 (NON-CURRENT)
Match location:
Position 1613 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GTACTTAAACAGTCTGCATAT
Hairpin Sequence:
5'-CCGG-GTACTTAAACAGTCTGCATAT-CTCGAG-ATATGCAGACTGTTTAAGTAC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGTACTTAAACAGTCTGCATATCTCGAGATATGCAGACTGTTTAAGTACTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGTACTTAAACAGTCTGCATATCTCGAGATATGCAGACTGTTTAAGTAC-3'

Other clones with same target sequence:

  1. TRCN0000075903