Construct: shRNA TRCN0000325960

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008881.1-1752s21c1
DNA Barcode:
CCTCTATGCTATGACGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxna1 (18844)
Gene Description:
plexin A1
Transcript:
RefSeq NM_008881.1 (NON-CURRENT)
Match location:
Position 1752 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000325960)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCTATGCTATGACGGAGAA
Hairpin Sequence:
5'-CCGG-CCTCTATGCTATGACGGAGAA-CTCGAG-TTCTCCGTCATAGCATAGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCTATGCTATGACGGAGAACTCGAGTTCTCCGTCATAGCATAGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCTATGCTATGACGGAGAACTCGAGTTCTCCGTCATAGCATAGAGG-3'

Other clones with same target sequence:

  1. TRCN0000079190