Construct: shRNA TRCN0000326263

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019552.2-1189s21c1
DNA Barcode:
GCTGTGATTTATGGGCGATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Abcb10 (56199)
Gene Description:
ATP-binding cassette, sub-family B (MDR/TAP), member 10
Transcript:
RefSeq NM_019552.2 (CURRENT)
Match location:
Position 1189 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326263)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGTGATTTATGGGCGATAT
Hairpin Sequence:
5'-CCGG-GCTGTGATTTATGGGCGATAT-CTCGAG-ATATCGCCCATAAATCACAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGTGATTTATGGGCGATATCTCGAGATATCGCCCATAAATCACAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGTGATTTATGGGCGATATCTCGAGATATCGCCCATAAATCACAGC-3'

Other clones with same target sequence:

  1. TRCN0000113446