Construct: shRNA TRCN0000326281

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_019832.3-571s21c1
DNA Barcode:
GCTGGAGTATGAAGAGCACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gkap1 (56278)
Gene Description:
G kinase anchoring protein 1
Transcript:
RefSeq NM_019832.3 (NON-CURRENT)
Match location:
Position 571 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326281)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGAGTATGAAGAGCACAA
Hairpin Sequence:
5'-CCGG-GCTGGAGTATGAAGAGCACAA-CTCGAG-TTGTGCTCTTCATACTCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGAGTATGAAGAGCACAACTCGAGTTGTGCTCTTCATACTCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGAGTATGAAGAGCACAACTCGAGTTGTGCTCTTCATACTCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000042794