Construct: shRNA TRCN0000326413

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010315.4-403s21c1
DNA Barcode:
AGCTGGTAGAACAGCTGAAGA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gng2 (14702)
Gene Description:
guanine nucleotide binding protein (G protein), gamma 2
Transcript:
RefSeq NM_010315.4 (CURRENT)
Match location:
Position 403 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326413)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCTGGTAGAACAGCTGAAGA
Hairpin Sequence:
5'-CCGG-AGCTGGTAGAACAGCTGAAGA-CTCGAG-TCTTCAGCTGTTCTACCAGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCTGGTAGAACAGCTGAAGACTCGAGTCTTCAGCTGTTCTACCAGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCTGGTAGAACAGCTGAAGACTCGAGTCTTCAGCTGTTCTACCAGCT-3'

Other clones with same target sequence:

  1. TRCN0000037056