Construct: shRNA TRCN0000326612

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008922.1-1128s21c1
DNA Barcode:
GCAGGAGTTTATCAGAGGAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prim2 (19076)
Gene Description:
DNA primase, p58 subunit
Transcript:
RefSeq NM_008922.1 (NON-CURRENT)
Match location:
Position 1128 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326612)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGGAGTTTATCAGAGGAAA
Hairpin Sequence:
5'-CCGG-GCAGGAGTTTATCAGAGGAAA-CTCGAG-TTTCCTCTGATAAACTCCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGGAGTTTATCAGAGGAAACTCGAGTTTCCTCTGATAAACTCCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGGAGTTTATCAGAGGAAACTCGAGTTTCCTCTGATAAACTCCTGC-3'

Other clones with same target sequence:

  1. TRCN0000111467