Construct: shRNA TRCN0000326687

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008619.1-2012s21c1
DNA Barcode:
GCAGCAATATCGGGTCTTAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mov10 (17454)
Gene Description:
Moloney leukemia virus 10
Transcript:
RefSeq NM_008619.1 (NON-CURRENT)
Match location:
Position 2012 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326687)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGCAATATCGGGTCTTAAT
Hairpin Sequence:
5'-CCGG-GCAGCAATATCGGGTCTTAAT-CTCGAG-ATTAAGACCCGATATTGCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGCAATATCGGGTCTTAATCTCGAGATTAAGACCCGATATTGCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGCAATATCGGGTCTTAATCTCGAGATTAAGACCCGATATTGCTGC-3'

Other clones with same target sequence:

  1. TRCN0000097833