Construct: shRNA TRCN0000326746

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133933.3-1066s21c1
DNA Barcode:
GCACTGAAGATGCGGTTTGTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rpn1 (103963)
Gene Description:
ribophorin I
Transcript:
RefSeq NM_133933.3 (NON-CURRENT)
Match location:
Position 1066 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326746)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACTGAAGATGCGGTTTGTA
Hairpin Sequence:
5'-CCGG-GCACTGAAGATGCGGTTTGTA-CTCGAG-TACAAACCGCATCTTCAGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACTGAAGATGCGGTTTGTACTCGAGTACAAACCGCATCTTCAGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACTGAAGATGCGGTTTGTACTCGAGTACAAACCGCATCTTCAGTGC-3'

Other clones with same target sequence:

  1. TRCN0000094042