Construct: shRNA TRCN0000326748

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_021327.1-2374s21c1
DNA Barcode:
CCTAAGAATGAGGTGCAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Tnip1 (57783)
Gene Description:
TNFAIP3 interacting protein 1
Transcript:
RefSeq NM_021327.1 (NON-CURRENT)
Match location:
Position 2374 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326748)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTAAGAATGAGGTGCAACAA
Hairpin Sequence:
5'-CCGG-CCTAAGAATGAGGTGCAACAA-CTCGAG-TTGTTGCACCTCATTCTTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTAAGAATGAGGTGCAACAACTCGAGTTGTTGCACCTCATTCTTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTAAGAATGAGGTGCAACAACTCGAGTTGTTGCACCTCATTCTTAGG-3'

Other clones with same target sequence:

  1. TRCN0000124114