Construct: shRNA TRCN0000326754

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001493.2-530s21c1
DNA Barcode:
CCCTGCTTCTGCTTTGACAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Wdr83os (414077)
Gene Description:
WD repeat domain 83 opposite strand
Transcript:
RefSeq NM_001001493.2 (CURRENT)
Match location:
Position 530 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326754)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCTGCTTCTGCTTTGACAAA
Hairpin Sequence:
5'-CCGG-CCCTGCTTCTGCTTTGACAAA-CTCGAG-TTTGTCAAAGCAGAAGCAGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCTGCTTCTGCTTTGACAAACTCGAGTTTGTCAAAGCAGAAGCAGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCTGCTTCTGCTTTGACAAACTCGAGTTTGTCAAAGCAGAAGCAGGG-3'

Other clones with same target sequence:

  1. TRCN0000124084