Construct: shRNA TRCN0000326857

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_025441.2-120s21c1
DNA Barcode:
GCTAGGAATGAGAGTAAACAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nemf (66244)
Gene Description:
nuclear export mediator factor
Transcript:
RefSeq NM_025441.2 (NON-CURRENT)
Match location:
Position 120 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326857)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTAGGAATGAGAGTAAACAA
Hairpin Sequence:
5'-CCGG-GCTAGGAATGAGAGTAAACAA-CTCGAG-TTGTTTACTCTCATTCCTAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTAGGAATGAGAGTAAACAACTCGAGTTGTTTACTCTCATTCCTAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTAGGAATGAGAGTAAACAACTCGAGTTGTTTACTCTCATTCCTAGC-3'

Other clones with same target sequence:

  1. TRCN0000150512
  2. TRCN0000127452