Construct: shRNA TRCN0000326883

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011276.3-1424s21c1
DNA Barcode:
GAGTACTATCAGGATTCCTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rlim (19820)
Gene Description:
ring finger protein, LIM domain interacting
Transcript:
RefSeq NM_011276.3 (CURRENT)
Match location:
Position 1424 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326883)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGTACTATCAGGATTCCTAT
Hairpin Sequence:
5'-CCGG-GAGTACTATCAGGATTCCTAT-CTCGAG-ATAGGAATCCTGATAGTACTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGTACTATCAGGATTCCTATCTCGAGATAGGAATCCTGATAGTACTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGTACTATCAGGATTCCTATCTCGAGATAGGAATCCTGATAGTACTC-3'

Other clones with same target sequence:

  1. TRCN0000095743