Construct: shRNA TRCN0000326942

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018830.1-838s21c1
DNA Barcode:
GCGTGGAACTATGTATGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Asah2 (54447)
Gene Description:
N-acylsphingosine amidohydrolase 2
Transcript:
RefSeq NM_018830.1 (CURRENT)
Match location:
Position 838 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000326942)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGTGGAACTATGTATGATTT
Hairpin Sequence:
5'-CCGG-GCGTGGAACTATGTATGATTT-CTCGAG-AAATCATACATAGTTCCACGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGTGGAACTATGTATGATTTCTCGAGAAATCATACATAGTTCCACGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGTGGAACTATGTATGATTTCTCGAGAAATCATACATAGTTCCACGC-3'

Other clones with same target sequence:

  1. TRCN0000124730