Construct: shRNA TRCN0000326976
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_028811.2-1584s21c1
- DNA Barcode:
- GCACAAGAAATTATTACCGAA
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Elp3 (74195)
- Gene Description:
- elongator acetyltransferase complex subunit 3
- Transcript:
- RefSeq NM_028811.2 (NON-CURRENT)
- Match location:
- Position 1584 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000326976)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- GCACAAGAAATTATTACCGAA
- Hairpin Sequence:
-
5'-CCGG-GCACAAGAAATTATTACCGAA-CTCGAG-TTCGGTAATAATTTCTTGTGC-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGGCACAAGAAATTATTACCGAACTCGAGTTCGGTAATAATTTCTTGTGCTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAGCACAAGAAATTATTACCGAACTCGAGTTCGGTAATAATTTCTTGTGC-3'
Other clones with same target sequence:
-
TRCN0000039311
