Construct: shRNA TRCN0000327085

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026623.3-610s21c1
DNA Barcode:
CCTCCTCAGTATCCGTATATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nudt21 (68219)
Gene Description:
nudix (nucleoside diphosphate linked moiety X)-type motif 21
Transcript:
RefSeq NM_026623.3 (CURRENT)
Match location:
Position 610 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327085)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCCTCAGTATCCGTATATT
Hairpin Sequence:
5'-CCGG-CCTCCTCAGTATCCGTATATT-CTCGAG-AATATACGGATACTGAGGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCCTCAGTATCCGTATATTCTCGAGAATATACGGATACTGAGGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCCTCAGTATCCGTATATTCTCGAGAATATACGGATACTGAGGAGG-3'

Other clones with same target sequence:

  1. TRCN0000109258