Construct: shRNA TRCN0000327191

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009702.2-4635s21c1
DNA Barcode:
GCCTGGGACATTCCATTTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Aqr (11834)
Gene Description:
aquarius
Transcript:
RefSeq NM_009702.2 (NON-CURRENT)
Match location:
Position 4635 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327191)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCTGGGACATTCCATTTATA
Hairpin Sequence:
5'-CCGG-GCCTGGGACATTCCATTTATA-CTCGAG-TATAAATGGAATGTCCCAGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCTGGGACATTCCATTTATACTCGAGTATAAATGGAATGTCCCAGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCTGGGACATTCCATTTATACTCGAGTATAAATGGAATGTCCCAGGC-3'

Other clones with same target sequence:

  1. TRCN0000123834