Construct: shRNA TRCN0000327268

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_010046.2-997s21c1
DNA Barcode:
GCCCTTCAAGGATATGGACTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Dgat1 (13350)
Gene Description:
diacylglycerol O-acyltransferase 1
Transcript:
RefSeq NM_010046.2 (NON-CURRENT)
Match location:
Position 997 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327268)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCCCTTCAAGGATATGGACTA
Hairpin Sequence:
5'-CCGG-GCCCTTCAAGGATATGGACTA-CTCGAG-TAGTCCATATCCTTGAAGGGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCCCTTCAAGGATATGGACTACTCGAGTAGTCCATATCCTTGAAGGGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCCCTTCAAGGATATGGACTACTCGAGTAGTCCATATCCTTGAAGGGC-3'

Other clones with same target sequence:

  1. TRCN0000124792