Construct: shRNA TRCN0000327307

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133758.2-1384s21c1
DNA Barcode:
GCTGGATATGAGCACATTTAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Usp47 (74996)
Gene Description:
ubiquitin specific peptidase 47
Transcript:
RefSeq NM_133758.2 (NON-CURRENT)
Match location:
Position 1384 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327307)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCTGGATATGAGCACATTTAT
Hairpin Sequence:
5'-CCGG-GCTGGATATGAGCACATTTAT-CTCGAG-ATAAATGTGCTCATATCCAGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCTGGATATGAGCACATTTATCTCGAGATAAATGTGCTCATATCCAGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCTGGATATGAGCACATTTATCTCGAGATAAATGTGCTCATATCCAGC-3'

Other clones with same target sequence:

  1. TRCN0000030871