Construct: shRNA TRCN0000327354

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133826.4-1716s21c1
DNA Barcode:
CTATGGTAATTCCTGGGAGAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1h (108664)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit H
Transcript:
RefSeq NM_133826.4 (NON-CURRENT)
Match location:
Position 1716 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327354)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATGGTAATTCCTGGGAGAA
Hairpin Sequence:
5'-CCGG-CTATGGTAATTCCTGGGAGAA-CTCGAG-TTCTCCCAGGAATTACCATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATGGTAATTCCTGGGAGAACTCGAGTTCTCCCAGGAATTACCATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATGGTAATTCCTGGGAGAACTCGAGTTCTCCCAGGAATTACCATAG-3'

Other clones with same target sequence:

  1. TRCN0000101450