Construct: shRNA TRCN0000327355

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133826.4-1059s21c1
DNA Barcode:
CTGGCTATGATTCAATGCAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp6v1h (108664)
Gene Description:
ATPase, H+ transporting, lysosomal V1 subunit H
Transcript:
RefSeq NM_133826.4 (NON-CURRENT)
Match location:
Position 1059 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327355)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTGGCTATGATTCAATGCAAA
Hairpin Sequence:
5'-CCGG-CTGGCTATGATTCAATGCAAA-CTCGAG-TTTGCATTGAATCATAGCCAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTGGCTATGATTCAATGCAAACTCGAGTTTGCATTGAATCATAGCCAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTGGCTATGATTCAATGCAAACTCGAGTTTGCATTGAATCATAGCCAG-3'

Other clones with same target sequence:

  1. TRCN0000101453