Construct: shRNA TRCN0000327425

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009728.2-1213s21c1
DNA Barcode:
CCTCGTTTGTATATCTCTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atp10a (11982)
Gene Description:
ATPase, class V, type 10A
Transcript:
RefSeq NM_009728.2 (CURRENT)
Match location:
Position 1213 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327425)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTCGTTTGTATATCTCTATT
Hairpin Sequence:
5'-CCGG-CCTCGTTTGTATATCTCTATT-CTCGAG-AATAGAGATATACAAACGAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTCGTTTGTATATCTCTATTCTCGAGAATAGAGATATACAAACGAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTCGTTTGTATATCTCTATTCTCGAGAATAGAGATATACAAACGAGG-3'

Other clones with same target sequence:

  1. TRCN0000101496