Construct: shRNA TRCN0000327521

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026842.3-1528s21c1
DNA Barcode:
CGGTTCTAATTGCCATATAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Ubqln1 (56085)
Gene Description:
ubiquilin 1
Transcript:
RefSeq NM_026842.3 (NON-CURRENT)
Match location:
Position 1528 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327521)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGTTCTAATTGCCATATAAA
Hairpin Sequence:
5'-CCGG-CGGTTCTAATTGCCATATAAA-CTCGAG-TTTATATGGCAATTAGAACCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGTTCTAATTGCCATATAAACTCGAGTTTATATGGCAATTAGAACCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGTTCTAATTGCCATATAAACTCGAGTTTATATGGCAATTAGAACCG-3'

Other clones with same target sequence:

  1. TRCN0000087733