Construct: shRNA TRCN0000327645

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_015850.3-3899s21c1
DNA Barcode:
AGTGGCTTATTAATTCCGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGFR1 (2260)
Gene Description:
fibroblast growth factor receptor 1
Transcript:
RefSeq NM_015850.3 (NON-CURRENT)
Match location:
Position 3899 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327645)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTGGCTTATTAATTCCGATA
Hairpin Sequence:
5'-CCGG-AGTGGCTTATTAATTCCGATA-CTCGAG-TATCGGAATTAATAAGCCACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTGGCTTATTAATTCCGATACTCGAGTATCGGAATTAATAAGCCACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTGGCTTATTAATTCCGATACTCGAGTATCGGAATTAATAAGCCACT-3'

Other clones with same target sequence:

  1. TRCN0000121307