Construct: shRNA TRCN0000327666

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_002883.2-1834s21c1
DNA Barcode:
CAAGAGTGAAGACAAGGTCAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
RANGAP1 (5905)
Gene Description:
Ran GTPase activating protein 1
Transcript:
RefSeq NM_002883.2 (NON-CURRENT)
Match location:
Position 1834 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327666)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGAGTGAAGACAAGGTCAA
Hairpin Sequence:
5'-CCGG-CAAGAGTGAAGACAAGGTCAA-CTCGAG-TTGACCTTGTCTTCACTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGAGTGAAGACAAGGTCAACTCGAGTTGACCTTGTCTTCACTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGAGTGAAGACAAGGTCAACTCGAGTTGACCTTGTCTTCACTCTTG-3'

Other clones with same target sequence:

  1. TRCN0000047332