Construct: shRNA TRCN0000327749

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014014.2-942s21c1
DNA Barcode:
GCAGATGAAGTATTGGAGATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SNRNP200 (23020)
Gene Description:
small nuclear ribonucleoprotein U5 subunit 200
Transcript:
RefSeq NM_014014.2 (NON-CURRENT)
Match location:
Position 942 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327749)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGATGAAGTATTGGAGATT
Hairpin Sequence:
5'-CCGG-GCAGATGAAGTATTGGAGATT-CTCGAG-AATCTCCAATACTTCATCTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGATGAAGTATTGGAGATTCTCGAGAATCTCCAATACTTCATCTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGATGAAGTATTGGAGATTCTCGAGAATCTCCAATACTTCATCTGC-3'

Other clones with same target sequence:

  1. TRCN0000051831