Construct: shRNA TRCN0000327765

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_006223.2-313s21c1
DNA Barcode:
CCGCACAGTATAGTGAAGATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIN4 (5303)
Gene Description:
peptidylprolyl cis/trans isomerase, NIMA-interacting 4
Transcript:
RefSeq NM_006223.2 (NON-CURRENT)
Match location:
Position 313 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327765)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCGCACAGTATAGTGAAGATA
Hairpin Sequence:
5'-CCGG-CCGCACAGTATAGTGAAGATA-CTCGAG-TATCTTCACTATACTGTGCGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCGCACAGTATAGTGAAGATACTCGAGTATCTTCACTATACTGTGCGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCGCACAGTATAGTGAAGATACTCGAGTATCTTCACTATACTGTGCGG-3'

Other clones with same target sequence:

  1. TRCN0000049218