Construct: shRNA TRCN0000327841

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_017637.5-2805s21c1
DNA Barcode:
CGTAAACTGTTGACCAAAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BNC2 (54796)
Gene Description:
basonuclin 2
Transcript:
RefSeq NM_017637.5 (NON-CURRENT)
Match location:
Position 2805 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327841)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGTAAACTGTTGACCAAAGAA
Hairpin Sequence:
5'-CCGG-CGTAAACTGTTGACCAAAGAA-CTCGAG-TTCTTTGGTCAACAGTTTACG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGTAAACTGTTGACCAAAGAACTCGAGTTCTTTGGTCAACAGTTTACGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGTAAACTGTTGACCAAAGAACTCGAGTTCTTTGGTCAACAGTTTACG-3'

Other clones with same target sequence:

  1. TRCN0000108178