Construct: shRNA TRCN0000327864

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018133.3-2177s21c1
DNA Barcode:
CCTTGCTACTCTAACCGCAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MSL2 (55167)
Gene Description:
MSL complex subunit 2
Transcript:
RefSeq NM_018133.3 (NON-CURRENT)
Match location:
Position 2177 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327864)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTTGCTACTCTAACCGCAAA
Hairpin Sequence:
5'-CCGG-CCTTGCTACTCTAACCGCAAA-CTCGAG-TTTGCGGTTAGAGTAGCAAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTTGCTACTCTAACCGCAAACTCGAGTTTGCGGTTAGAGTAGCAAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTTGCTACTCTAACCGCAAACTCGAGTTTGCGGTTAGAGTAGCAAGG-3'

Other clones with same target sequence:

  1. TRCN0000037033