Construct: shRNA TRCN0000327962

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_009499.2-623s21c1
DNA Barcode:
AGGAGGACGCCATACAGTTTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vasp (22323)
Gene Description:
vasodilator-stimulated phosphoprotein
Transcript:
RefSeq NM_009499.2 (NON-CURRENT)
Match location:
Position 623 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000327962)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAGGACGCCATACAGTTTG
Hairpin Sequence:
5'-CCGG-AGGAGGACGCCATACAGTTTG-CTCGAG-CAAACTGTATGGCGTCCTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAGGACGCCATACAGTTTGCTCGAGCAAACTGTATGGCGTCCTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAGGACGCCATACAGTTTGCTCGAGCAAACTGTATGGCGTCCTCCT-3'

Other clones with same target sequence:

(none)