Construct: shRNA TRCN0000328084
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_010587.2-3395s21c1
- DNA Barcode:
- CTAACTATGTGAGGCTTAAAG
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Itsn1 (16443)
- Gene Description:
- intersectin 1 (SH3 domain protein 1A)
- Transcript:
- RefSeq NM_010587.2 (CURRENT)
- Match location:
- Position 3395 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000328084)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTAACTATGTGAGGCTTAAAG
- Hairpin Sequence:
-
5'-CCGG-CTAACTATGTGAGGCTTAAAG-CTCGAG-CTTTAAGCCTCACATAGTTAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTAACTATGTGAGGCTTAAAGCTCGAGCTTTAAGCCTCACATAGTTAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTAACTATGTGAGGCTTAAAGCTCGAGCTTTAAGCCTCACATAGTTAG-3'
Other clones with same target sequence:
-
TRCN0000233549
