Construct: shRNA TRCN0000328136

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_053226.1-203s21c1
DNA Barcode:
TCATAGCTGTGGACATGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Vmn1r53 (113853)
Gene Description:
vomeronasal 1 receptor 53
Transcript:
RefSeq NM_053226.1 (NON-CURRENT)
Match location:
Position 203 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328136)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCATAGCTGTGGACATGTTTA
Hairpin Sequence:
5'-CCGG-TCATAGCTGTGGACATGTTTA-CTCGAG-TAAACATGTCCACAGCTATGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCATAGCTGTGGACATGTTTACTCGAGTAAACATGTCCACAGCTATGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCATAGCTGTGGACATGTTTACTCGAGTAAACATGTCCACAGCTATGA-3'

Other clones with same target sequence:

(none)