Construct: shRNA TRCN0000328161
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- NM_053158.2-610s21c1
- DNA Barcode:
- CTGGTTAAGAAGATCATGGAT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Mrpl1 (94061)
- Gene Description:
- mitochondrial ribosomal protein L1
- Transcript:
- RefSeq NM_053158.2 (NON-CURRENT)
- Match location:
- Position 610 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000328161)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- CTGGTTAAGAAGATCATGGAT
- Hairpin Sequence:
-
5'-CCGG-CTGGTTAAGAAGATCATGGAT-CTCGAG-ATCCATGATCTTCTTAACCAG-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGCTGGTTAAGAAGATCATGGATCTCGAGATCCATGATCTTCTTAACCAGTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAACTGGTTAAGAAGATCATGGATCTCGAGATCCATGATCTTCTTAACCAG-3'
Other clones with same target sequence:
-
TRCN0000176699
