Construct: shRNA TRCN0000328218

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_172703.2-2309s21c1
DNA Barcode:
GGGCTCACCTTCCCGTTTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Eif4g3 (230861)
Gene Description:
eukaryotic translation initiation factor 4 gamma, 3
Transcript:
RefSeq NM_172703.2 (NON-CURRENT)
Match location:
Position 2309 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328218)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGCTCACCTTCCCGTTTAAA
Hairpin Sequence:
5'-CCGG-GGGCTCACCTTCCCGTTTAAA-CTCGAG-TTTAAACGGGAAGGTGAGCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGCTCACCTTCCCGTTTAAACTCGAGTTTAAACGGGAAGGTGAGCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGCTCACCTTCCCGTTTAAACTCGAGTTTAAACGGGAAGGTGAGCCC-3'

Other clones with same target sequence:

(none)