Construct: shRNA TRCN0000328454

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_011870.4-602s21c1
DNA Barcode:
CTTTGCCAGCTCCTTTAAGAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cib1 (23991)
Gene Description:
calcium and integrin binding 1 (calmyrin)
Transcript:
RefSeq NM_011870.4 (NON-CURRENT)
Match location:
Position 602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328454)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTTGCCAGCTCCTTTAAGAT
Hairpin Sequence:
5'-CCGG-CTTTGCCAGCTCCTTTAAGAT-CTCGAG-ATCTTAAAGGAGCTGGCAAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTTGCCAGCTCCTTTAAGATCTCGAGATCTTAAAGGAGCTGGCAAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTTGCCAGCTCCTTTAAGATCTCGAGATCTTAAAGGAGCTGGCAAAG-3'

Other clones with same target sequence:

  1. TRCN0000120586