Construct: shRNA TRCN0000328487

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_008882.2-6776s21c1
DNA Barcode:
CTATTCCCTTCTTAGTAATAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Plxna2 (18845)
Gene Description:
plexin A2
Transcript:
RefSeq NM_008882.2 (CURRENT)
Match location:
Position 6776 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328487)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTATTCCCTTCTTAGTAATAT
Hairpin Sequence:
5'-CCGG-CTATTCCCTTCTTAGTAATAT-CTCGAG-ATATTACTAAGAAGGGAATAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTATTCCCTTCTTAGTAATATCTCGAGATATTACTAAGAAGGGAATAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTATTCCCTTCTTAGTAATATCTCGAGATATTACTAAGAAGGGAATAG-3'

Other clones with same target sequence:

(none)