Construct: shRNA TRCN0000328638

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198862.2-2145s21c1
DNA Barcode:
GCAGTATCTGCACATAGGCTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Nlgn2 (216856)
Gene Description:
neuroligin 2
Transcript:
RefSeq NM_198862.2 (CURRENT)
Match location:
Position 2145 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328638)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCAGTATCTGCACATAGGCTT
Hairpin Sequence:
5'-CCGG-GCAGTATCTGCACATAGGCTT-CTCGAG-AAGCCTATGTGCAGATACTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCAGTATCTGCACATAGGCTTCTCGAGAAGCCTATGTGCAGATACTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCAGTATCTGCACATAGGCTTCTCGAGAAGCCTATGTGCAGATACTGC-3'

Other clones with same target sequence:

  1. TRCN0000184441