Construct: shRNA TRCN0000328682

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_023042.2-784s21c1
DNA Barcode:
ATGACTTCAGGCCTGATTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Recql (19691)
Gene Description:
RecQ protein-like
Transcript:
RefSeq NM_023042.2 (NON-CURRENT)
Match location:
Position 784 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328682)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACTTCAGGCCTGATTATA
Hairpin Sequence:
5'-CCGG-ATGACTTCAGGCCTGATTATA-CTCGAG-TATAATCAGGCCTGAAGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACTTCAGGCCTGATTATACTCGAGTATAATCAGGCCTGAAGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACTTCAGGCCTGATTATACTCGAGTATAATCAGGCCTGAAGTCAT-3'

Other clones with same target sequence:

(none)