Construct: shRNA TRCN0000328756

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175383.2-1167s21c1
DNA Barcode:
ACGAACGCTTTCGGCAGTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4gat1 (108902)
Gene Description:
beta-1,4-glucuronyltransferase 1
Transcript:
RefSeq NM_175383.2 (CURRENT)
Match location:
Position 1167 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328756)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACGAACGCTTTCGGCAGTATG
Hairpin Sequence:
5'-CCGG-ACGAACGCTTTCGGCAGTATG-CTCGAG-CATACTGCCGAAAGCGTTCGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACGAACGCTTTCGGCAGTATGCTCGAGCATACTGCCGAAAGCGTTCGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACGAACGCTTTCGGCAGTATGCTCGAGCATACTGCCGAAAGCGTTCGT-3'

Other clones with same target sequence:

(none)