Construct: shRNA TRCN0000328813

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_175383.2-311s21c1
DNA Barcode:
CGATCCGTAGACCAGGTAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
B4gat1 (108902)
Gene Description:
beta-1,4-glucuronyltransferase 1
Transcript:
RefSeq NM_175383.2 (CURRENT)
Match location:
Position 311 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328813)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGATCCGTAGACCAGGTAAAG
Hairpin Sequence:
5'-CCGG-CGATCCGTAGACCAGGTAAAG-CTCGAG-CTTTACCTGGTCTACGGATCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGATCCGTAGACCAGGTAAAGCTCGAGCTTTACCTGGTCTACGGATCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGATCCGTAGACCAGGTAAAGCTCGAGCTTTACCTGGTCTACGGATCG-3'

Other clones with same target sequence:

(none)