Construct: shRNA TRCN0000328925

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_026474.5-969s21c1
DNA Barcode:
CGCAGCTTTAAACAAGTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Sugt1 (67955)
Gene Description:
SGT1, suppressor of G2 allele of SKP1 (S. cerevisiae)
Transcript:
RefSeq NM_026474.5 (CURRENT)
Match location:
Position 969 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000328925)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCAGCTTTAAACAAGTTATT
Hairpin Sequence:
5'-CCGG-CGCAGCTTTAAACAAGTTATT-CTCGAG-AATAACTTGTTTAAAGCTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCAGCTTTAAACAAGTTATTCTCGAGAATAACTTGTTTAAAGCTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCAGCTTTAAACAAGTTATTCTCGAGAATAACTTGTTTAAAGCTGCG-3'

Other clones with same target sequence:

(none)